Genetic Murine Models in Tuberculosis Research

TSC1^f/f-ERCre⁺ mice were previously reported [27 (link), 56 (link)]. LysM-Cre⁺ mice were purchased from Jackson Laboratory. M. bovis (Bacillus Calmette-Guérin or BCG) was obtained from the laboratory of Dr. William Jacobs (Albert Einstein College of Medicine). Virulent M. tuberculosis H37Rv (ATCC, 25618D-2) was purchased from ATCC. Rapamycin was obtained from Enzo Life Science (Enzo Life Science, A275). Bafilomycin A1 (B1793), 3-methyladenine (3-MA, M9281), and wortmannin (W1628) were purchased from Sigma-Aldrich. Compound C (Millipore, 171,260) was obtained from Millipore (Billerica, MA).

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Pan H., Zhong X.P, & Lee S. (2016). Sustained activation of mTORC1 in macrophages increases AMPKα-dependent autophagy to maintain cellular homeostasis. BMC Biochemistry, 17, 14.

Publication 2016

LysM-Cre+ mice Rapamycin Bafilomycin A1 (B1793), wortmannin (W1628) Compound C

3 methyladenine Bacillus calmette guérin Bafilomycin a1 M tuberculosis h37rv Medicine Mice Rapamycin Wortmannin

Corresponding Organization :

Other organizations : Duke University, Duke University Hospital, Duke Medical Center

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Variable analysis

independent variables

Genotype: TSC1^f/f-ERCre⁺ mice
Genotype: LysM-Cre⁺ mice
Bacterial strain: Bacillus Calmette-Guérin (BCG) or Mycobacterium tuberculosis H37Rv
Drug treatment: Rapamycin, Bafilomycin A1, 3-methyladenine (3-MA), Wortmannin, Compound C

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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