Example 1
Antibodies to CD59 and K41 Amadori-modified GCD59 were each generated using CD59 fragments. For anti-K41 Amadori-modified GCD59 antibody preparation, CD59 fragments having Amadori-modified K41 were used. Antibodies were prepared by mouse immunization and development of hybridoma cells from animals exhibiting successful expression of antibodies with high affinity and specificity. Clone H9 was developed as a capture antibody, binding to both glycated and non-glycated CD59. Clones D2 and D3 were developed as detection antibodies, binding to K41 Amadori-modified GCD59. Antibodies were sequenced and analyzed to identify antibody regions. Resulting sequences are provided in Tables 1-7. Antibodies D2 and D3 were found to have heavy and light chains with identical amino acid sequences, but with heavy chain nucleotide sequences differing by a single nucleotide.
