Live-cell Imaging of Whole Neurons

Live-cell visualization of whole neurons was performed by transfection (Bakkum et al., 2013 (link); Radivojevic et al., 2016 (link)). Transfection was performed using a pLV-hSyn-RFP plasmid from Edward Callaway (The Salk Institute, US; Addgene, #22909) and Lipofectamine 2000 (Life Technologies) in accordance with the manufacturer's protocol. A Leica DM6000 FS microscope, a Leica DFC 345 FX camera, and the Leica Application Suite software were used to produce the micrographs.

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Bullmann T., Radivojevic M., Huber S.T., Deligkaris K., Hierlemann A, & Frey U. (2019). Large-Scale Mapping of Axonal Arbors Using High-Density Microelectrode Arrays. Frontiers in Cellular Neuroscience, 13, 404.

Publication 2019

Lipofectamine 2000 DM6000 FS microscope DFC 345 FX camera

Cell Lipofectamine 2000 Microscope Neurons Plasmid Transfection

Corresponding Organization : ETH Zurich

Other organizations : Kyoto College of Graduate Studies for Informatics, Kyoto University, Leipzig University, RIKEN Center for Computational Science, Ube Frontier University, Osaka University, Maxwell Technologies (Switzerland)

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Variable analysis

independent variables

Transfection using a pLV-hSyn-RFP plasmid and Lipofectamine 2000

dependent variables

Live-cell visualization of whole neurons

control variables

Manufacturer's protocol for Lipofectamine 2000 transfection

controls

No positive or negative controls were explicitly mentioned in the provided information.

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