Influenza Virus Infection in BALB/c Mice

Six-week-old female BALB/c mice (Beijing Experimental Animal Center, Beijing, China) were lightly anesthetized with CO₂ and inoculated i.n. with 10⁶ 50% egg infective doses (EID₅₀) of H3N2 avian influenza virus in a volume of 50 μL. Control mice were inoculated with 50 μL PBS. On day 3 post-inoculation (p.i.), three mice in each group were euthanized and their organs, including brains, nasal turbinates, spleens, kidneys and lungs, were collected for virus titration. The remaining five mice were monitored daily for weight loss and mortality for the following 2 weeks. The histological study of the lung sample was performed as described previously [9 (link)]. A monoclonal antibody against the NP protein of A/duck/Zhejiang/11/2000(H5N1) virus and rabbit anti-mouse IgG as the secondary antibody (Sigma-Aldrich) were used in the immunohistochemical (IHC) assays.

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Guan L., Shi J., Kong X., Ma S., Zhang Y., Yin X., He X., Liu L., Suzuki Y., Li C., Deng G, & Chen H. (2019). H3N2 avian influenza viruses detected in live poultry markets in China bind to human-type receptors and transmit in guinea pigs and ferrets. Emerging Microbes & Infections, 8(1), 1280-1290.

Publication 2019

rabbit anti-mouse IgG

A protein Anti igg Antibody Assays Avian influenza a virus Balb c mice Brains Duck Experimental animal Female H5n1 virus Inoculation Kidneys Lung Monoclonal antibody Mouse Nasal turbinates Rabbit Titration Virus

Corresponding Organization :

Other organizations : Harbin Veterinary Research Institute, Chubu University

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Variable analysis

independent variables

Inoculation with 10^6 50% egg infective doses (EID50) of H3N2 avian influenza virus in a volume of 50 μL

dependent variables

Virus titers in organs (brains, nasal turbinates, spleens, kidneys, and lungs) on day 3 post-inoculation (p.i.)
Weight loss and mortality of mice for 2 weeks post-inoculation
Histological changes in the lung samples

control variables

Age (six-week-old) and sex (female) of BALB/c mice
Route of inoculation (intranasal, i.n.)
Anesthesia method (lightly anesthetized with CO2)

controls

Positive control: None specified
Negative control: Mice inoculated with 50 μL PBS

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