Cell Line Propagation and Fingerprinting

All cell lines were propagated at 37°C and 5% CO₂ in humidified atmosphere. MCF10A cells were obtained from the ATCC and cultured as described previously (24 (link)). HMLER cells were provided by S. Mani (MD Anderson Cancer Center, Houston, TX) and maintained in MEGM medium (Lonza) without serum and antibiotics. Parental Ba/F3 cells were cultured in RPMI1640 medium supplemented with final concentration of 5% fetal bovine serum and 2.5 ng/ml recombinant mouse IL3 (R&D Systems). Lentivirus production and cell transduction were described earlier (25 (link),26 (link)). Cells lines were fingerprinted prior to use on April 21, 2015 by the MD Anderson Cancer Center Characterized Cell Line Core using STR testing platform. Ba/F3 is a mouse-originated cell line, thus STR testing could not be performed.