Real-time PCR for 14-3-3ζ and USP18 expression

Real-time PCR (qRT-PCR) assays were performed as before.^{21 (link)} Primers were human 14-3-3ζ primer (Hs00237047_m1, Thermo Fisher Scientific, Waltham, MA) and murine 14-3-3ζ primer (Mm01158417_g1, Thermo Fisher Scientific, Waltham, MA); human USP18 primer (Hs00276441_m1, Thermo Fisher Scientific, Waltham, MA) and murine USP18 primer (Mm01188805_m1, Thermo Fisher Scientific, Waltham, MA); human actin primer (Hs01060665_g1, Thermo Fisher Scientific, Waltham, MA); and murine actin primer (Mm01205647_g1, Thermo Fisher Scientific, Waltham, MA).

Free full text: Click here

Chen Z., Zheng L., Chen Y., Liu X., Kawakami M., Mustachio L.M., Roszik J., Ferry-Galow K.V., Parchment R.E., Liu X., Andresson T., Duncan G., Kurie J.M., Rodriguez-Canales J., Liu X, & Dmitrovsky E. (2022). Loss of ubiquitin-specific peptidase 18 destabilizes 14-3-3ζ protein and represses lung cancer metastasis. Cancer Biology & Therapy, 23(1), 265-280.

Publication 2022

Hs01060665_g1

Actin Assays Human Murine Primer Real time pcr Usp18

Corresponding Organization : Frederick National Laboratory for Cancer Research

Other organizations : AstraZeneca (United States)

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Human 14-3-3ζ primer
Murine 14-3-3ζ primer
Human USP18 primer
Murine USP18 primer
Human actin primer
Murine actin primer

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!