Cardiac Function Assessment in Mice

Echocardiography and hemodynamic measurements were performed at the indicated times after the mice were anesthetized using 1.5–2% isoflurane3 (link)5 (link)6 (link)22 (link)37 (link)44 (link)45 (link). For echocardiography, M-mode images derived from the short axis of the LV were recorded using a Mylab30CV (ESAOTE) machine with a 15-MHz probe. To measure the LVEDD, LVESD and LVFS (LVFS (%)=(LVEDD-LVESD)/LVEDD × 100%), M-mode measurements of the LV internal diameter were obtained from at least three beats and then averaged. Cardiac catheterization studies were performed using a 1.4-French Millar catheter-tip micromanometer catheter (SPR-839; Millar Instruments), which was inserted through the right carotid artery into the left ventricle. The pressure and dp/dt were recorded continuously with an Aria pressure-volume conductance system coupled to a Powerlab/4SP A/D converter and were stored and displayed on a personal computer.

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Jiang D.S., Wei X., Zhang X.F., Liu Y., Zhang Y., Chen K., Gao L., Zhou H., Zhu X.H., Liu P.P., Bond Lau W., Ma X., Zou Y., Zhang X.D., Fan G.C, & Li H. (2014). IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling. Nature Communications, 5, 3303.

Publication 2014

Mylab30CV

Aria Axis Cardiac catheterization Carotid artery Catheter Echocardiography Hemodynamic Left ventricle Lvfs Mice Pressure

Corresponding Organization :

Other organizations : Wuhan University, Huazhong University of Science and Technology, University of Ottawa, Thomas Jefferson University, Fudan University, University of Cincinnati

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Variable analysis

independent variables

Anesthesia using 1.5–2% isoflurane

dependent variables

Echocardiographic measurements (LVEDD, LVESD, LVFS)
Hemodynamic measurements (pressure and dp/dt)

control variables

Anesthesia using 1.5–2% isoflurane
Insertion of Millar catheter-tip micromanometer catheter through the right carotid artery into the left ventricle

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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