Murine Islet Dissociation and Tunicamycin Treatment
Corresponding Organization :
Other organizations : University of Colorado Denver
Variable analysis
- Treatment with 100 µM tauroursodeoxycholic acid (TUDCA)
- Addition of 1 µg/mL tunicamycin
- Cell antigen response
- Islet cells from NOD mice
- Cell dissociation using non-enzymatic cell dissociation buffer
- Cell culture in DMEM/10% FBS low-glucose media (1 g/L glucose)
- Overnight incubation at 37°C
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