Oligosaccharide Quantification Protocol

The distribution and quantification of oligosaccharides in the fractions were determined as previously reported [12 (link)]. The analysis was conducted using HPLC equipped with a Sugar-D column (4.6 mm × 250 mm, NACALAI TESQUE, INC., Kyoto, Japan) with a column oven temperature at 40 °C. The products were eluted with an isocratic elution system of acetonitrile/water (4:1, v/v) at a flow rate of 1.0 mL/min, and the products were detected with a refractive index detector. X1, X2, X3, and DX3 were used as standards.

Free full text: Click here

Mune Mune M.A., Hatanaka T., Kishimura H, & Kumagai Y. (2024). Understanding Antidiabetic Potential of Oligosaccharides from Red Alga Dulse Devaleraea inkyuleei Xylan by Investigating α-Amylase and α-Glucosidase Inhibition. Molecules, 29(7), 1536.

Publication 2024

Sugar-D column

Corresponding Organization :

Other organizations : University of Maroua, Hokkaido University

Top 5 similar protocols

Variable analysis

independent variables

Column type (Sugar-D column, 4.6 mm × 250 mm, NACALAI TESQUE, INC., Kyoto, Japan)
Column oven temperature (40 °C)
Elution system (acetonitrile/water, 4:1, v/v)
Flow rate (1.0 mL/min)

dependent variables

Distribution and quantification of oligosaccharides in the fractions

control variables

Elution system (isocratic)

controls

X1, X2, X3, and DX3 were used as standards

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!