For studies with MC38 tumors, wild type C57BL/6 mice were implanted with MC38 cells as described above. When tumor volume reached an average of 75 mm3, mice were randomized into groups of n = 15 mice. For studies with E0771 tumors, Ptpn6fl/fl mice were implanted as described above. For anti-PD1 studies: mice were dosed intraperitoneally (IP) with anti-PD1 (clone RMP1-14, BioXCell) or rat IgG2a isotype control (clone 2A3, BioXCell) at 10 mg/kg twice weekly (BIW). For anti-CD47 studies, mice were dosed IP with anti-CD47 (clone miap301, BioXCell) or rat IgG2a isotype control (clone 2A3, BioXCell) at 20 mg/kg BIW for 2 weeks as described in Liu et al. (41 (link)).
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