SFB-ZRANB1 and its deletion-mutant-containing expression vectors were described in our previous publication (Zhang et al., 2018a (link)). pCLXSU (GFP)-HA-ZRANB1 and its C443A mutation were gifts from Shao-Cong Sun’s laboratory (Houston, TX, USA) stock as described previously (Jin et al., 2016 (link)). Full-length ZRANB1, which was obtained from the HA-ZRANB1 expression vector, was subcloned into the pCDH-MYC-S-tag and pCDH-FLAG vectors, and its C443A mutation was subcloned into the pCDH-MYC-S-tag vector. The SLC7A11-FLAG-HA-pHAGE vector was obtained from Alex Toker’s lab (Boston, MA, USA; Lien et al., 2017 (link)). Full-length SLC7A11 was subcloned into the pBabe-SFB and pLenti-V5 vectors using the Gateway system (Invitrogen) or into the pCDH-MYC-S-tag and pHAGE-MYC vectors using the HiFi system (NEB; New England Biolabs). pRK5-HA-ubiquitin was obtained from Addgene (plasmid number: 17608). The SLC7A11 shRNAs were subcloned into pLKO vectors and the sequences were as follows: shSLC-1:5′-CCGGGCTCTCATTTAAGGTTCCCTTCTCGAGAAGGGAACCTTAAATGAGAGCTTTTTG-3′; shSLC-2: 5′-CCG​GCC​TAC​ATC​ATC​GGT​ACT​TCA​ACT​CGA​GTT​GAA​GTA​CCG​ATG​ATG​TAG​GTT​TTT​G-3′.