Fetal Sex Determination via cfDNA

Maternal and, when available, paternal gDNA was extracted from whole blood on a MagnaPure platform (Roche) via a MagnaPure compact nucleic acid isolation kit I. If saliva from the father was available, gDNA was extracted with a MagnaPure compact nucleic acid isolation kit I, large volume, on the same platform.
Fetal cfDNA was extracted from maternal plasma on the aforementioned platform as well, also via the large volume kit. This cfDNA was used in a Y chromosome qPCR reaction so that the fetal sex could be determined on the basis of the detection two amplicons for DYS14 and SRY, as has been described.⁶ (link)^,⁹ (link)

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Vossaert L., Wang Q., Salman R., McCombs A.K., Patel V., Qu C., Mancini M.A., Edwards D.P., Malovannaya A., Liu P., Shaw C.A., Levy B., Wapner R.J., Bi W., Breman A.M., Van den Veyver I.B, & Beaudet A.L. (2019). Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis. American Journal of Human Genetics, 105(6), 1262-1273.

Publication 2019

MagnaPure platform MagnaPure compact nucleic acid isolation kit

Blood Cfdna Fetal Isolation Maternal Nucleic acid Plasma Saliva Y chromosome

Corresponding Organization : Texas Children's Hospital

Other organizations : Columbia University Irving Medical Center, Indiana University – Purdue University Indianapolis

Top 5 similar protocols

Variable analysis

independent variables

Maternal gDNA extraction method
Paternal gDNA extraction method
Fetal cfDNA extraction method

dependent variables

Fetal sex determination based on detection of DYS14 and SRY amplicons

control variables

Whole blood collection for maternal and paternal gDNA extraction
Maternal plasma collection for fetal cfDNA extraction
Use of MagnaPure platform and nucleic acid isolation kits for DNA/cfDNA extraction

positive controls

Detection of two amplicons for DYS14 and SRY to determine fetal sex

negative controls

Not explicitly mentioned

Annotations

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