Western Blot and Immunohistochemistry Protocol

Western blotting was performed as described previously [20 (link)]. Samples of equivalent total protein (20 μg) were loaded. Primary antibodies against CD63, CD9 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), c-MYC, p-EGFR, p-MEK, p-ERK(Cell Signaling Technology, Danvers, MA, USA), GAPDH, DNAJB11, HSPA5, ATF6, IRE1, XBP1, PERK, ATF4, EGFR, Raf-1, MEK, and ERK1/2 (ProteinTech Group, Rosemont, IL, USA) were used. Immunohistochemical (IHC) assay was performed following a previously described procedure [21 (link)].

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Liu P., Zu F., Chen H., Yin X, & Tan X. (2022). Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway. Cellular & Molecular Biology Letters, 27, 87.

Publication 2022

c-MYC p-EGFR p-MEK p-ERK GAPDH HSPA5 Raf-1 ERK1/2

Antibodies Assay Atf4 Atf6 C myc Egfr Erk1 Gapdh Hspa5 Protein Raf 1 Xbp1

Corresponding Organization : China Medical University

Other organizations : Shenyang Center for Disease Control and Prevention

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Variable analysis

independent variables

Primary antibodies against CD63, CD9, c-MYC, p-EGFR, p-MEK, p-ERK, GAPDH, DNAJB11, HSPA5, ATF6, IRE1, XBP1, PERK, ATF4, EGFR, Raf-1, MEK, and ERK1/2

dependent variables

Protein expression levels of CD63, CD9, c-MYC, p-EGFR, p-MEK, p-ERK, GAPDH, DNAJB11, HSPA5, ATF6, IRE1, XBP1, PERK, ATF4, EGFR, Raf-1, MEK, and ERK1/2

control variables

Total protein (20 μg) loaded per sample

controls

No positive or negative controls were explicitly mentioned.

Annotations

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