Cell Viability Assay for Compound Screening

Cells were plated at a density of 5000 cells in triplicates in a 96-well plate. On the next day, treatment of the cells was performed with the indicated compound/s at the specified concentrations in 100 μL media as a final volume. Cell viability was considered after 72 h using MTT solution (Promega, Madison, WI, USA) [28 (link)]. The reagent (20 μLs) was added to each well followed by incubation of the plate for 3 h and fluorescence was subsequently measured (570 nm) using a plate reader, then IC₅₀ values were assessed using the GraphPad prism 7 [29 (link),30 (link)].

Free full text: Click here

Eltamany E.E., Elhady S.S., Ahmed H.A., Badr J.M., Noor A.O., Ahmed S.A, & Nafie M.S. (2020). Chemical Profiling, Antioxidant, Cytotoxic Activities and Molecular Docking Simulation of Carrichtera annua DC. (Cruciferae). Antioxidants, 9(12), 1286.

Publication 2020

MTT solution GraphPad prism 7

Cell viability Cells Compound s Fluorescence Prism Promega

Corresponding Organization :

Other organizations : Suez Canal University, King Abdulaziz University

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

Compound/s at the specified concentrations

dependent variables

Cell viability

control variables

Cell density (5000 cells)
Number of replicates (triplicates)
Plate format (96-well plate)
Media volume (100 μL)
Incubation time (72 h)
MTT assay (Promega, Madison, WI, USA)
Fluorescence measurement (570 nm)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!