MCF10A (Human breast epithelial cells, ATCC, Manassas, VA, USA), MCF-7, and MDA-MB-231 (BC cells, Cell Bank of Chinese Academy of Science, Shanghai, China) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS, 100 units/mL penicillin and 100 mg/mL streptomycin (Invitrogen, Carlsbad, CA, USA) under condition of 5% CO2 flow at 37°C. DOX‐resistant MCF‐7 (MCF‐7/DOX) and DOX‐resistant MDA-MB-231 (MDA-MB-231/DOX) cells were generated by continuous exposure of MCF‐7 or MDA-MB-231 cells to gradient concentrations of DOX (Sigma‐Aldrich, St Louis, MO, USA) [19 (link)]. In addition, in this study, 1.5 μg/mL DOX was applied for the treatment of BC cells, and 30 μg/mL DOX was used to treat DOX-resistant BC cells.
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Breast Cancer Cell Line Maintenance and Treatment
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Corresponding Organization :
Other organizations : Hunan Cancer Hospital, Xiangya Hospital Central South University, Central South University
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Variable analysis
independent variables
- Doxorubicin (DOX) treatment
- Doxorubicin (DOX) resistance
- Cell growth and survival
- Cell culture medium (DMEM) supplemented with 10% FBS, 100 units/mL penicillin and 100 mg/mL streptomycin
- Incubation conditions (5% CO2 at 37°C)
- Positive control: MCF-7 and MDA-MB-231 breast cancer cell lines
- Negative control: Not explicitly mentioned
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