Diverse Human Cell Lines for Cancer Research

Human MM cell lines MSTO-211H (MSTO parent) and NCI-H226 were obtained from the American Type Culture Collection (ATCC, Rockville, MD). MSTO parent cells were stably transfected with a full-length human CD26 (MSTO-CD26) [6 (link)]. Human MM cell line JMN cells were transduced with the short hairpin RNA (shRNA)-expressing lentivirus, generating the stable cell lines JMN CD26-shRNA and JMN ctrl-shRNA [19 (link)]. For non-tumor human cells, immortalized pleural mesothelial cell line MeT-5A, mammary epithelial cell line MCF10A, fetal lung fibroblast cell line TIG-1, human umbilical vein endothelial cells (HUVEC), and human dermal microvascular endothelial cells (HDMVEC) were used. MeT-5A and MCF10A were obtained from ATCC. TIG-1 was obtained from JCRB Cell Bank (Osaka, Japan). HUVEC, HDMVEC and the culture media for MCF10A, HUVEC, HDMVEC (MEGM, EGM-2, EGM-2MV, respectively) were purchased from LONZA (Walkersville, MD). MSTO parent, MSTO-CD26, JMN ctrl-shRNA, JMN CD26-shRNA, H226 and MeT-5A were grown in RPMI 1640 medium supplemented with 10% FBS. TIG-1 was grown in DMEM medium supplemented with 10% FBS. All the cells were cultured at 37 °C in a humidified 5% CO₂ incubator.