In total, 5 ml of blood samples was collected aseptically and centrifuged to separate the serum within 3 h, and all serum specimens were evaluated within 24 h. The HBsAg, hepatitis B surface antibody (HBsAb), hepatitis B pre‐core antigen (HBeAg), hepatitis B pre‐core antibody (HBeAb), and hepatitis B core antibody (HBcAb) levels were evaluated using the Abbott i2000sr automatic immunoassay analyzer (Abbott Company, the USA). The markers of liver and kidney function were determined using AU5800 Automatic Analyzer (Beckman Coulter, Galway, Ireland). Complete blood count was evaluated using BC6600 plus Analyzer (Beckman Coulter, Galway, Ireland).
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