The antibacterial activity of the leaf extract and the biosynthesized AgNPs was assessed against Staphylococcus aureus (Gram-positive bacteria) and Xanthomonas spp. (Gram-negative bacteria) using the agar well diffusion method. Before use, the nutrient agar and petri dish were autoclaved. Then, 0.1 mL of pure bacterial culture was evenly spread on nutrient agar plates using an L-rod. Then, wells created by the well borer on agar plates were filled with 20 μL of aqueous samples of leaf extract and AgNPs (1000 ppm). Streptomycin and distilled water were employed as the positive and negative control, respectively. The plates were finally incubated at 37 °C for 24 h to obtain the results.
Using the agar well diffusion method, the antifungal activity of the leaf extract and the biosynthesized AgNPs were evaluated against Macrophomina phaseolina and Fusarium oxysporum. Before use, the potato dextrose agar and petri dish were autoclaved. Then, 0.1 mL of pure fungal culture was evenly spread on nutrient agar plates using an L-rod. The wells on the agar plates were then formed by the well borer, and 20 μL of aqueous samples of leaf extract and AgNPs (1000 ppm) was added. Nystatin and distilled water were employed as the positive and negative controls, respectively. The plates were finally incubated at 37 °C for 48 h to obtain the results.
Using the agar well diffusion method, the antifungal activity of the leaf extract and the biosynthesized AgNPs were evaluated against Macrophomina phaseolina and Fusarium oxysporum. Before use, the potato dextrose agar and petri dish were autoclaved. Then, 0.1 mL of pure fungal culture was evenly spread on nutrient agar plates using an L-rod. The wells on the agar plates were then formed by the well borer, and 20 μL of aqueous samples of leaf extract and AgNPs (1000 ppm) was added. Nystatin and distilled water were employed as the positive and negative controls, respectively. The plates were finally incubated at 37 °C for 48 h to obtain the results.
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