Quantification of Anthraquinones, Stilbenes, and Phenolic Compounds
Corresponding Organization : University of Łódź
Other organizations : Polish Academy of Sciences, Institute of Soil Science and Plant Cultivation
Protocol cited in 1 other protocol
Variable analysis
- Derivatives of anthraquinones (aloe-emodin, aloe-emodin-8-Glu, emodin, emodin-8-Glu, rhein, rhein-8-Glu, chrysophanol, chrysophanol-8-Glu, physcion, sennoside-A, sennoside-B, sennoside-C, sennoside-D)
- Stilbenes (resveratrol, pterostilbene, pinostilbene, piceatannol, astringin, polydatin, rhapontigenin, rhaponticin, isorhapontigenin, isorhaponticin, deoxyrhapontigenin, deoxyrhaponticin)
- Phenolic acids (glucogallin, gallic-acid)
- Flavones (vicenin-II, vicenin-III, apigenin-7-Glu)
- Flavanones (pinocembrin, pinocembroside)
- Chalcones (phloretin, phloridzin, trilobatin)
- Catechins (catechin, epicatechin, gallocatechin, procyanidin-B1, procyanidin-B2, procyanidin-B3, and procyanidin-C1)
- Not explicitly mentioned
- Acetonitrile LC-MS grade
- Formic acid MS-grade
tert -butanol- Methanol,
n -hexane, andn -butanol (all of analytical grade) - Ultrapure water prepared using a Milli-Q water purification system (MerckMillipore)
- General reagents for bioassays purchased from Sigma-Aldrich (a part of Merck KGaA, Darmstadt, Germany) and Cayman Chemicals (Ann Arbor, MI, USA)
- Reagents specific to cell culture, gene expression analyses, and cytokine profiling
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