Efavirenz, lopinavir and ritonavir reference compounds, and internal standards (IS), ritonavir-d6 and celecoxib, were obtained from Toronto Research Chemicals (Toronto, Canada). Proteinase K was purchased from Sigma™. Acetonitrile, methanol and other solvents or reagents were HPLC grade or analytical grade. Human hair samples were obtained from patients on lopinavir/ritonavir or efavirenz–based HAART in the Women’s Interagency HIV Study (WIHS). Blank human hair samples were obtained from healthy volunteers to serve as negative controls.
Protocol detail
Hair-Based Antiretroviral Quantification
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Acetonitrile
Celecoxib
Efavirenz
Haart
Hair
Healthy volunteers
Hplc
Human
Lopinavir
Lopinavir ritonavir
Methanol
Patients
Proteinase k
Ritonavir
Solvents
Women
Corresponding Organization : University of California, San Francisco
Top 5 similar protocols
Protocol cited in 15 other protocols
Variable analysis
independent variables
- Efavirenz
- Lopinavir
- Ritonavir
- Drug concentrations in human hair samples
- Ritonavir-d6 (internal standard)
- Celecoxib (internal standard)
- Proteinase K
- Acetonitrile, methanol and other solvents or reagents (HPLC grade or analytical grade)
- Blank human hair samples from healthy volunteers (negative controls)
- Positive control: Human hair samples from patients on lopinavir/ritonavir or efavirenz–based HAART
- Negative control: Blank human hair samples from healthy volunteers
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!