Mice naïve to behavioral testing were injected in alternating saline/CFA order. Mice were anesthetized with 2% isoflurane and sedation was confirmed by the absence of a reflex during a toe pinch. CFA (50 μL, Thermo Fisher), or sterile saline as a control, was injected into the plantar surface of the right hind-paw; this volume and unilateral strategy have previously been shown to induce thermal hyperalgesia and mechanical hypersensitivity persisting for weeks [14 (link),34 (link)–36 (link),54 (link),66 (link)]. Comparison of individual mice injected with saline or CFA was used to determine the behavioral alteration induced by inflammatory pain.
Protocol detail
Unilateral Inflammatory Pain Induction
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Hypersensitivity
Inflammatory
Isoflurane
Mice
Pain
Reflex
Saline
Sedation
Sterile
Thermal hyperalgesia
Corresponding Organization :
Other organizations : Washington University in St. Louis
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Variable analysis
independent variables
- Injection of CFA (50 μL, Thermo Fisher) or sterile saline as a control
- Thermal hyperalgesia
- Mechanical hypersensitivity
- Unilateral injection strategy (into the plantar surface of the right hind-paw)
- Anesthesia with 2% isoflurane and confirmation of sedation by the absence of a reflex during a toe pinch
- Positive control: Injection of CFA to induce inflammatory pain
- Negative control: Injection of sterile saline
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