For in vitro inhibition tests of oligomerization/ fibrillation, we used lyophilized amyloid-β 1–40 (Aβ40) and lyophilized Methionine amyloid-β 1–40 (MAβ40) [33 (link)] which were bought from rPeptide LLC (Bogart, GA, USA).
For sample preparation, the first weighing of amyloid-β (Aβ) with a high precision balance was completed, then Hexafluoroisopropanol (HFIP; Sigma Aldrich Company) was added to achieve 5–20 μM solutions for concentration adjustments related to pre-measurements of the peptide quality. Furthermore, such treated Aβ were pipetted into marked tubes, vacuum evaporated for 24 h and finally frozen at –80°C. These samples were stored at the same temperature for a minimum of one day but less than two weeks until usage [34–37 (link)].
For sample preparation, the first weighing of amyloid-β (Aβ) with a high precision balance was completed, then Hexafluoroisopropanol (HFIP; Sigma Aldrich Company) was added to achieve 5–20 μM solutions for concentration adjustments related to pre-measurements of the peptide quality. Furthermore, such treated Aβ were pipetted into marked tubes, vacuum evaporated for 24 h and finally frozen at –80°C. These samples were stored at the same temperature for a minimum of one day but less than two weeks until usage [34–37 (link)].
