Western Blot Analysis of Protein Markers

WB was performed as previously described 17 (link). After incubation with the secondary antibody, the proteins were detected by chemiluminescence (Millipore Germany). The primary antibodies used in this study were anti-LHX6 (1:1000; Santa Cruz Biotechnology), anti-CTNNB1 (1:700; Santa Cruz Biotechnology), anti-MMP7 (1:1000; Abcam), anti-c-Myc (1:1000; Santa Cruz Biotechnology), anti-CCND1 (1:1000; Santa Cruz Biotechnology) and anti-GAPDH (1:2000; Beyotime China).

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Yang J., Han F., Liu W., Zhang M., Huang Y., Hao X., Jiang X., Yin L., Chen H., Cao J., Zhang H, & Liu J. (2017). LHX6, An Independent Prognostic Factor, Inhibits Lung Adenocarcinoma Progression through Transcriptional Silencing of β-catenin. Journal of Cancer, 8(13), 2561-2574.

Publication 2017

anti-CTNNB1 anti-MMP7 anti-c-Myc anti-CCND1

Antibodies Antibody Ccnd1 Chemiluminescence Ctnnb1 Gapdh Mmp7 Proteins

Corresponding Organization :

Other organizations : Army Medical University, Yan'an Hospital Affiliated To Kunming Medical University, Kunming Medical University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein levels of LHX6, CTNNB1, MMP7, c-Myc, CCND1 detected by Western blot

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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