Constructing Consensus Genetic Map

In order to increase the number of loci mapped and get more precision about their localization in our consensus map, we used six different recombinant inbred line (RIL) populations (supporting information, Table S1). We used data obtained for three RIL populations previously described: Térèse × K586 (Pop1, Laucou et al. 1998 ), Térèse × Champagne (Pop2, Loridon et al. 2005 (link)) and Caméor× China (Pop9, Deulvot et al. 2010 (link)). Furthermore, Cameor, VavD265, Ballet, three pea (Pisum sativum L.) lines showing variability in protein content and seed protein composition, were selected to create interconnected RIL populations by single seed descent from the crosses between Cameor × VavD265 (Pop3, 211 F6:8), Cameor × Ballet (Pop4, 207 F6:8), Ballet × VavD265 (Pop5, 211 F6:8). Leaf tissues were harvested from the F6 plant and then from a bulk of eight F7 or F8 plants for further DNA extractions. Total DNA was extracted from leaf tissues according to Dellaporta et al. (1983) . A total of 1022 lines were used to build the consensus map.

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Bordat A., Savois V., Nicolas M., Salse J., Chauveau A., Bourgeois M., Potier J., Houtin H., Rond C., Murat F., Marget P., Aubert G, & Burstin J. (2011). Translational Genomics in Legumes Allowed Placing In Silico 5460 Unigenes on the Pea Functional Map and Identified Candidate Genes in Pisum sativum L.. G3: Genes|Genomes|Genetics, 1(2), 93-103.

Publication 2011

Bulk Leaf Pisum sativum Plant Plants dna Populations Protein Tissues

Corresponding Organization :

Other organizations : Institut National de la Recherche Agronomique

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Variable analysis

independent variables

Six different recombinant inbred line (RIL) populations

dependent variables

Number of loci mapped
Localization precision of loci in consensus map

control variables

Leaf tissues were harvested from the F6 plant and then from a bulk of eight F7 or F8 plants for further DNA extractions
Total DNA was extracted from leaf tissues according to Dellaporta et al. (1983)

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