pCW-FLAG-WT, Y537S, or D538G were created from pCW-Cas9 [62 (link)] (Addgene). pFLAG-CMV constructs were made from pFLAG-CMV2 (Sigma). YFP-tagged ESR1-YAP1 was constructed by PCR of the ESR1-YAP1 open reading frame (ORF) [11 (link)], followed by ligation into digested pECFP-C1 (Clontech). pBIND-YAP1 (GAL4 DBD-YAP1 amino acid 230–504 fusion expression plasmid) was constructed by ligation into pBIND (Promega). All constructs were sequenced.
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