Comprehensive Immune Cell Phenotyping

Spleens and adipose tissue (pooled gonadal/renal fat pads) were harvested and processed as described (5 (link), 6 (link)). Cells were stained with antibodies, then results were acquired using a BD LSR II (BD Biosciences) and analyzed with FlowJo software (Tree Star). CD19-BV510 and Vβ8.3-PE (BD Biosciences). I-A^d-A488, CD8α-A700, CD4-APC, CD11c-APC/Cy7, CD3ε-PE, CD11b-PE/Cy7, CD54-FITC, CD40-PE, H²K^d-FITC, I-A^d-PE, CD11c-biotin, SA-APC/Cy7 and TruStain FcX (BioLegend). CD86-APC (eBioscience).

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Boi S.K., Buchta C.M., Pearson N.A., Francis M.B., Meyerholz D.K., Grobe J.L, & Norian L.A. (2016). Obesity alters immune and metabolic profiles: new insight from obese-resistant mice on high fat diet. Obesity (Silver Spring, Md.), 24(10), 2140-2149.

Publication 2016

BD LSR II CD4-APC CD11c-APC/Cy7 CD3ε-PE CD11b-PE/Cy7 CD54-FITC CD40-PE H2Kd-FITC CD11c-biotin SA-APC/Cy7 TruStain FcX CD86-APC

Adipose tissue Antibodies Biotin Cd11b Cd3ε Cd54 Cells Fat pads Fitc Gonadal Renal Tree

Corresponding Organization : University of Alabama at Birmingham

Other organizations : University of Iowa, Fraternal Order of Eagles

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Variable analysis

independent variables

Spleens and adipose tissue (pooled gonadal/renal fat pads)

dependent variables

Cells stained with antibodies
Results acquired using a BD LSR II (BD Biosciences) and analyzed with FlowJo software (Tree Star)

control variables

Independent variables not explicitly mentioned.
Control variables not explicitly mentioned.

controls

Positive controls: Not specified.
Negative controls: Not specified.

Annotations

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