Lymphocytes isolated from wild-type (WT) mouse spleens were labeled with TAMRA (Invitrogen) as previously described.24 (link) Briefly, re-suspended lymphocytes were pre-warmed for 15 min at 37 °C in a water bath, mixed with TAMRA and incubated for another 12 min. Labeling effectiveness was checked by flow cytometry analysis. TAMRA-labeled lymphocytes were transferred intravenously into BALT-bearing mice via the tail vein. Mice were sacrificed 18 h later, and lungs were collected and analyzed by LiSM.
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Tracking Lymphocyte Migration in BALT
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Other organizations : Medizinische Hochschule Hannover, University of Veterinary Medicine Hannover, Foundation, Center for Experimental and Clinical Infection Research, Helmholtz Centre for Infection Research, Ludwig-Maximilians-Universität München
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Variable analysis
independent variables
- Labeling lymphocytes with TAMRA
- Lymphocyte labeling effectiveness (checked by flow cytometry analysis)
- Lymphocyte migration and localization in the lungs (analyzed by LiSM)
- Wild-type (WT) mouse spleens
- Pre-warming lymphocytes at 37 °C for 15 min
- Incubating lymphocytes with TAMRA for 12 min
- Transferring TAMRA-labeled lymphocytes intravenously into BALT-bearing mice
- Sacrificing mice 18 h after lymphocyte transfer and collecting lungs
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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