Liposomal Cytochalasin B Preparation
Corresponding Organization :
Other organizations : Syracuse University
Protocol cited in 1 other protocol
Variable analysis
- Lipid composition (egg phosphatidylcholine, or a 70:30 mixture of egg phosphatidylcholine and dimyristoylphosphatidylglycerol)
- Formation of large, unilamellar vesicles
- Amount of cytochalasin B (2.0 mg)
- Volume of chloroform (2.0 ml)
- Amount of egg phosphatidylcholine (200 mg)
- Volume of phosphate buffered saline (5 ml)
- Extrusion through a 5.0 μm polycarbonate filter (five times)
- Incubation time (1 h)
- Positive control: Liposomal cytochalasin B prepared using egg phosphatidylcholine
- Negative control: Not mentioned
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