Liposomal Cytochalasin B Preparation

Liposomal cytochalasin B was prepared by suspending 2.0 mg cytochalasin B in 2.0 ml chloroform containing 200 mg of egg phosphatidylcholine (Sigma-Aldrich Corp.). The solution was pipetted into a round bottom flask and dried to a thin film. The film was then resuspended in 5 ml phosphate buffered saline (Sigma-Aldrich Corp). The resulting suspension was left to sit for 1 h before being extruded five times through a 5.0 μm polycarbonate filter in accordance to LUVET (large unilamellar vesicles made by an extrusion technique), forming large, unilamellar vesicles. The above mentioned protocol was then repeated using 200 mg total of a 70:30 mixture of egg phosphatidylcholine: dimyristoylphosphatidylglycerol.