Berries Toxicity Evaluation in HUVECs

In HUVECs, the toxicity of the berries solutions was assessed by lactate dehydrogenase (LDH) release. The enzyme LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH; next the diaphorase reduces tetrazolium salt, oxidizing NADH, to a red formazan product that can be spectrophotometrically determined at 490 nm. LDH release from HUVECs treated for 24 h with berries solutions was monitored by collecting aliquots of the medium under the same experimental conditions performed above for cell viability assays, as previously reported [35 (link)]. The decrease in absorbance between the treatment after 24 h and the control was monitored at 37 °C at 490 nm using an AMR-100 Microplate reader (Allsheng).