For macrophage depletion experiments, mice bearing KPC1245 tumors were treated with 50 mg/kg of anti-CSF1R antibody (clone AFS 98, BioXcell), administered intraperitoneally, every alternate day, until tumors were harvested on day 15. For CD8 depletion experiments, mice with KPC1245 tumors were treated with 200 μg of anti-CD8 (clone YTS169.4) or an isotype rat IgG2b control (LTF-2) from Bio-X-Cell administered intraperitoneally on day −3, 0, 3, 6, and 10 of tumor inoculation as described previously (20 (link)).
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Macrophage and CD8+ T Cell Depletion in Murine Tumor Models
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Other organizations : University of California, San Diego
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Variable analysis
independent variables
- Anti-CSF1R antibody (clone AFS 98, BioXcell) administered intraperitoneally every alternate day
- Anti-CD8 (clone YTS169.4) or an isotype rat IgG2b control (LTF-2) from Bio-X-Cell administered intraperitoneally on day -3, 0, 3, 6, and 10 of tumor inoculation
- Tumor growth/size
- Mouse model with KPC1245 tumors
- Positive control: Anti-CD8 (clone YTS169.4)
- Negative control: Isotype rat IgG2b control (LTF-2)
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