CA LNCaP, Du145, and PC3 cells were obtained from ATCC, while E006AA and E006AA-hT, AA prostate cancer cell lines were established and characterised by Dr Koochekpour's laboratory (Koochekpour et al, 2004 (link), 2014 (link)). Cells were maintained as recommended by ATCC. Primary antibodies against HSP10 and cell cycle and apoptotic cocktail (pCdk/pHH3/Actin/PARP), anti-MMP-9, anti-MMP-2 were from Abcam, MA, USA; anti-HSP60 was from Chemicon; anti-HSP90 from R&D Systems USA, anti-HSP70 from Stressgen, USA; and anti-actin antibody was from M.P. Biomedical USA. Rabbit and mouse HRP-labelled secondary antibodies were procured from GE Healthcare Life Sciences, Pittsburgh, PA, USA. All other reagents were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Protocol detail
Characterization of Prostate Cancer Cell Lines
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Actin
Anti antibody
Anti d
Antibodies
Apoptotic
Cell cycle
Cell lines
Cells
Hsp70
Hsp90
Mmp 2
Mmp 9
Mouse
Pc3 cells
Prostate cancer
Rabbit
Corresponding Organization :
Other organizations : Roswell Park Comprehensive Cancer Center, University of Georgia
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Variable analysis
independent variables
- Cell lines: CA LNCaP, Du145, PC3, E006AA, E006AA-hT
- Expression of HSP10, HSP60, HSP70, HSP90, cell cycle and apoptotic markers (pCdk, pHH3, Actin, PARP), MMP-9, MMP-2
- Cell culture conditions as recommended by ATCC
- Positive control: Not specified
- Negative control: Not specified
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