To further confirmed the role of oxidative stress on endothelial dysfunction caused by H. pylori infection, the antioxidant NAC was used to treat the mice in vivo and endothelial cells in vitro. NAC is an FDA-approved drug and has been traditionally considered an antioxidant that effectively attenuates ROS production (15 (link)).
Mice in the NAC treatment group received NAC (Sigma-Aldrich, MO, United States, 1 mg/ml in drinking water) 3 days before the first gavage until the end of the experiment (16 (link)). NAC was changed every other day and covered with aluminum foil to avoid exposure to direct light. For the in vitro study, endothelial cells were incubated with 10 mM NAC as described (17 (link)).
Mice in the NAC treatment group received NAC (Sigma-Aldrich, MO, United States, 1 mg/ml in drinking water) 3 days before the first gavage until the end of the experiment (16 (link)). NAC was changed every other day and covered with aluminum foil to avoid exposure to direct light. For the in vitro study, endothelial cells were incubated with 10 mM NAC as described (17 (link)).
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