Trilineage Differentiation of Adipose Stem Cells

To investigate the trilineage differentiation potential, ASCs-_EXP and ASCs-_SVF of abd adipose tissue and ASCs-_EXP of rb and sc adipose tissue were initially expanded to P5 or P6 and then induced to adipogenic, osteogenic and chondrogenic differentiation in two experimental runs (n = 6). In all experiments, negative controls (NC) were included. The medium was changed every two to three days. In the case of adipogenic and osteogenic differentiation, cell culture vessels were coated with 2 µg/cm² murine laminin (Sigma Aldrich, Taufkirchen, Germany) in order to avoid cell detachment as far as possible, which was observed in Trachsel et al. due to the absence of any coating [59 (link)]. To ensure reproducibility, experiments were carried out in duplicate.