Fluorojade B staining was performed to assess SE-induced neuronal death (Mouri et al., 2008 (link)). Briefly, brain slices were cryosectioned at a thickness of 12 μm and tissue was fixed in formalin. Slices were passed through graded ethanol, incubated in a 0.006% potassium permanganate and finally incubated in 0.001% Fluorojade B. Neuronal damage was assessed by manual counting of Fluorojade B positive cells in the CA1, CA2, CA3 and dentate gyrus hippocampal regions under a Leica DM4000 epifluorescence microscope.
Brindley E., Heiland M., Mooney C., Diviney M., Mamad O., Hill T.D., Yan Y., Venø M.T., Reschke C.R., Batool A., Langa E., Sanz-Rodriguez A., Heller J.P., Morris G., Conboy K., Kjems J., Brennan G.P, & Henshall D.C. (2023). Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsy. Frontiers in Molecular Neuroscience, 16, 1230942.
Other organizations :
Royal College of Surgeons in Ireland, University College Dublin, Aarhus University, Dublin City University, Manchester Academic Health Science Centre, University of Manchester, University College London
Neuronal damage assessed by manual counting of Fluorojade B positive cells in the CA1, CA2, CA3 and dentate gyrus hippocampal regions
control variables
Brain slices cryosectioned at a thickness of 12 μm
Tissue fixed in formalin
Slices passed through graded ethanol
Slices incubated in a 0.006% potassium permanganate
Slices incubated in 0.001% Fluorojade B
controls
No positive or negative controls were explicitly mentioned in the provided information.
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