Western Blot Analysis of LV Proteins

Western blot analysis was performed on flash-frozen LV tissue samples as previously described (26 (link), 32 (link)). Briefly, we extracted protein from LV tissues and performed immunoblotting for various proteins using the following primary antibodies: SERCA2a, NCX1 (Thermo Fisher Scientific), PLN-P^Ser16, total PLN (Badrilla Ltd), Akt-P^Ser473, Akt-P^Thr308, total Akt (Cell Signaling), AMPK-P^Thr172 and total AMPK (Cell Signaling) and subsequently incubated with HRP conjugated secondary antibodies respectively.

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Zhabyeyev P., Sadasivan C., Shah S., Wang F, & Oudit G.Y. (2023). Amlodipine rescues advanced iron overload cardiomyopathy in hemojuvelin knockout murine model: Clinical implications. Frontiers in Cardiovascular Medicine, 10, 1129349.

Publication 2023

SERCA2a Akt-PSer473 Akt-PThr308 total Akt AMPK-PThr172 total AMPK

Antibodies Frozen Proteins Tissues Western blot analysis

Corresponding Organization :

Other organizations : University of Alberta

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Variable analysis

independent variables

SERCA2a
PLN-P^Ser16
Total PLN
Akt-P^Ser473
Akt-P^Thr308
Total Akt
AMPK-P^Thr172
Total AMPK

dependent variables

Protein expression levels of various proteins measured using Western blot analysis

control variables

LV tissue samples
Protein extraction from LV tissues
Immunoblotting procedure
HRP conjugated secondary antibodies

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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