Epididymis adipose tissue (EAT) were obtained freshly. The EAT were then washed with PBS containing 1% Penicillin–Streptomycin (Thermo Fisher) and cut into small pieces. DMEM/F12 (Procell) were used as tissue culture medium containing 1 mM sodium pyruvate, 2 mM glutamine, MEM vitamins, MEM nonessential amino acids as previously described [28 (link)]. 48 h doxorubicin (0.2 mM, MCE) treatment were used to induce senescence. For senolytic treatment, quercetin (20 µM) and dasatinib (1 µM) were added into culture medium for 48 h.
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