DNA and Surface Labeling of P. falciparum IEs

P. falciparum IEs were DNA labeled with ethidium bromide and surface labeled with rat anti-HB3VAR21-DBLβ_D4 (1:20) and fluorescein isothiocyanate (FITC)-conjugated secondary rabbit anti-rat IgG (1:150; Vector Labs) as described previously (50 (link)). Fluorescence data from ethidium bromide-positive cells were collected on an FC500 MPL flow cytometer (Beckman Coulter) and analyzed using WinList, version 9.0 (Verity Software House).

Free full text: Click here

Olsen R.W., Ecklu-Mensah G., Bengtsson A., Ofori M.F., Kusi K.A., Koram K.A., Hviid L., Adams Y, & Jensen A.T. (2019). Acquisition of IgG to ICAM-1-Binding DBLβ Domains in the Plasmodium falciparum Erythrocyte Membrane Protein 1 Antigen Family Varies between Groups A, B, and C. Infection and Immunity, 87(10), e00224-19.

Publication 2019

FITC)-conjugated secondary rabbit anti-rat IgG FC500 MPL WinList, version 9.0

Anti igg Cells Ethidium bromide Fluorescein Fluorescence Isothiocyanate Rabbit Vector

Corresponding Organization :

Other organizations : University of Copenhagen, Noguchi Memorial Institute for Medical Research, University of Ghana, Copenhagen University Hospital, Rigshospitalet

Top 5 similar protocols

Variable analysis

independent variables

P. falciparum IEs

dependent variables

Fluorescence data from ethidium bromide-positive cells

control variables

Rat anti-HB3VAR21-DBLβ_D4 (1:20) and fluorescein isothiocyanate (FITC)-conjugated secondary rabbit anti-rat IgG (1:150; Vector Labs)

controls

Positive control: None specified
Negative control: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!