After the virus injections, an optical fiber was positioned in a ceramic ferrule (230 μm in diameter, Xi 'an Bogao Optoelectronic Technology Co., LTD) and inserted into the regions of M1 and 5Cb for fiber photometry. The signals were collected by a laser beam through a 488 nm simulator (OBIS 488LS; Coherent), reflected off a dichroic mirror (MD498; Thorlabs), focused by an objective lens (Olympus, Japan), and coupled through a fiber collimation package (F240FC-A, Thorlabs) into a patch cable. The cable was connected to fibers chronically implanted in the mouse. Fluorescent signals were bandpass filtered (MF525-39, Thorlabs) and collected by a photomultiplier tube (CMOS, IDS imaging).
As described in previous studies 25 (link), 26 , the photometry data were imported to MATLAB R2020b MAT files for further analysis. The data was smoothed with a moving average filter and segmented based on behavioral events. The values of fluorescence change (ΔF/F) were calculated by (F-F0)/F0, where F0 is the baseline fluorescence signal averaged over a 1.5 s control time window. ΔF/F values were illustrated by spectrogram or average plots with a shaded area indicating SEM.
As described in previous studies 25 (link), 26 , the photometry data were imported to MATLAB R2020b MAT files for further analysis. The data was smoothed with a moving average filter and segmented based on behavioral events. The values of fluorescence change (ΔF/F) were calculated by (F-F0)/F0, where F0 is the baseline fluorescence signal averaged over a 1.5 s control time window. ΔF/F values were illustrated by spectrogram or average plots with a shaded area indicating SEM.
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