MTS Assay for Cell Viability

The cell survival was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)- 2(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Sigma). For the MTS assay (Promega), 1 × 10⁴ cells per well from each cell line were seeded triplicately in a 96-well plate. At 1st d, 2nd d,3rd d, 4th d and 5th day, 20 μL of MTS was added to each well and incubated for 1 h at 37 °C; the results were analyzed by a plate reader at 490 nm. The sample data were normalized to the background readings of media only [16 (link)].

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Yao T., Weng X., Yao Y., Huang C., Li J., Peng Y., Lin R, & Lin Z. (2020). ALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer. BMC Cancer, 20, 891.

Publication 2020

MTS) assay

Assay Cell line Cell survival Cells Promega Tetrazolium

Corresponding Organization :

Other organizations : Sun Yat-sen Memorial Hospital, Sun Yat-sen University

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Variable analysis

independent variables

Number of days (1st, 2nd, 3rd, 4th, 5th)

dependent variables

Cell survival assessed using MTS assay

control variables

Cell seeding density (1 × 10^4 cells per well)
Measurement time (1 h incubation with MTS)
Measurement method (plate reader at 490 nm)
Background readings (media only)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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