Caspase-3/7 Activity Quantification

Caspase-3/7 activity was evaluated immediately after the cell viability determination in the same wells using a synthetic rhodamine-labeled caspase-3/7 substrate (Apo-ONE^® Homogeneous Caspase-3/7 Assay, G7790; Promega Corporation). After 60 minutes of cultivation at the room temperature, the fluorescence was measured (ex: 499 nm/em: 512 nm) with an FL600 fluorescence plate reader (Bio-Tek). Caspase-3/7 activity was calculated as the fluorescence readings of the treated group/mock control ×100.33 (link)

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Zhang Y., Zhang L.J., Dang Y.W., Li S.H., Yan H.B, & Chen G. (2018). Clinical significance and effect of MTDH/AEG-1 in bladder urothelial cancer: a study based on immunohistochemistry, RNA-seq, and in vitro verification. Cancer Management and Research, 10, 6921-6936.

Publication 2018

Apo-ONE® Homogeneous Caspase-3/7 Assay FL600 fluorescence plate reader

Apo 3 Assay Caspase Caspase 7 Cell viability Fluorescence Promega Rhodamine

Corresponding Organization :

Other organizations : First Affiliated Hospital of GuangXi Medical University, Guangxi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Caspase-3/7 activity

control variables

Cell viability determination
Cultivation time (60 minutes)
Cultivation temperature (room temperature)
Fluorescence measurement (ex: 499 nm/em: 512 nm)

controls

Mock control

Annotations

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