Oregano Seed Extraction and Fractionation

The ethanol extract and ethyl acetate fraction of oregano seed used in the experiment were prepared in the same manner as in the research method by Lee et al. (2021 (link)). Ethanol (80%) per 100 g of oregano seed (Turkey) was added 15 times and mixed for 6 h. The solid was then filtered, concentrated under reduced pressure, and freeze‐dried to prepare an ethanol extract. The freeze‐dried ethanol extract was fractionated in the order of hexane, ethyl acetate, butanol, and water to prepare the ethyl acetate fraction of the oregano seed.

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Lee H., Bae J., Park K.W, & Kim M. (2024). Ethyl acetate fraction of oregano seed protects non‐alcoholic fatty liver in high‐fat diet‐induced obese mice through modulation of Srebp‐1c. Food Science & Nutrition, 12(4), 2578-2587.

Publication 2024

Corresponding Organization :

Other organizations : Kangwon National University, Sungkyunkwan University

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