Breast Cancer Cell Line MCF7 Treatment Protocols

MCF7 breast cancer cell line was obtained from ATCC. MCF7 cells were grown in DMEM medium, with 10% fetal bovine serum (FBS), penicillin (100 U/mL) and streptomycin (100 µg/mL). Cells were plated 24 h before treatment with different drugs at the indicated concentrations.^{27 (link)} Recombinant human heregulin (HRG) and IGF1 were obtained from Sigma Chemical Co (St. Louis, MO).^{27 (link)} PRCP inhibitor (PRCP-7414); catalog number 504044 was from Calbiochem. OSI906 and lapatinib are obtained from Selleckchem.

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Duan L., Calhoun S.J., Perez R.E., Macias V., Mir F., Gattuso P, & Maki C.G. (2022). Prolylcarboxypeptidase promotes IGF1R/HER3 signaling and is a potential target to improve endocrine therapy response in estrogen receptor positive breast cancer. Cancer Biology & Therapy, 23(1), 1-10.

Publication 2022

MCF7 breast cancer cell line IGF1 OSI906 lapatinib

Cells Drugs Fetal bovine serum Heregulin Human Igf1 Lapatinib Mcf7 cells Osi906 Penicillin Streptomycin

Corresponding Organization : Rush University Medical Center

Other organizations : University of Illinois at Chicago

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Variable analysis

independent variables

Treatment with different drugs at the indicated concentrations

dependent variables

Cell response to different drug treatments

control variables

MCF7 breast cancer cell line obtained from ATCC
Cells grown in DMEM medium with 10% fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 µg/mL)
Cells plated 24 h before treatment

controls

Positive control: Recombinant human heregulin (HRG) and IGF1 obtained from Sigma Chemical Co (St. Louis, MO)
Negative control: Not explicitly mentioned

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