Extracellular β-glucosidase activity was measured according to the methods reported previously [20 (link)-
Extracellular β-Glucosidase Activity Assay
Extracellular β-glucosidase activity was measured according to the methods reported previously [20 (link)-
Corresponding Organization :
Other organizations : University of Illinois Urbana-Champaign, Chonnam National University
Variable analysis
- Cell concentration monitoring method (UV-visible spectrophotometer)
- Glucose, cellobiose, cellodextrin and ethanol analysis method (HPLC with RI detector)
- Extracellular β-glucosidase activity measurement method
- Cell concentration (measured by OD at 600 nm)
- Glucose, cellobiose, cellodextrin and ethanol concentrations
- Extracellular β-glucosidase activity (units of β-glucosidase per liter and per gram of dry cell)
- HPLC column (Rezex ROA-Organic Acid H+ (8%) column)
- HPLC column elution conditions (0.005 N of H2SO4 at a flow rate of 0.6 ml/min at 50°C)
- Positive control: Not specified
- Negative control: Not specified
Annotations
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