Apoptosis and Necrosis Detection in U87MG Cells

In this experiment, a population of apoptotic and necrotic cells of the U87MG cell line was detected using an Annexin V–fluorescein isothiocyanate (FITC)/propidium iodide (PI) (BD Biosciences, San Jose, CA, USA) detection kit according to the manufacturer’s protocol. Briefly, the tested U87MG cells were plated into 6-well culture dishes (2 × 10⁵ cells/well) for 24 h prior to the addition of hairy root extract of S. obtusifolia at the IC₅₀ concentration used in this study. Following 24 h incubation with both extracts, the percentage of apoptotic/necrotic cells was determined by the Annexin V–FITC/PI assay, according to our previous studies [60 (link)].