Transcriptional Profiling of MgtS in E. coli

MG1655 ΔmgtS araE^con (kan) (GSO818) cells harboring pBAD24 or pBAD24-MgtS grown to OD₆₀₀ ~0.5 in LB were induced with arabinose for 7.5 min, after which cells were harvested and total RNA was prepared as described previously (Durand & Storz, 2010 (link)). The preparation of the cDNA and hybridization to the Affymetrix E. coli Genome 2.0 array were performed as described in Affymetrix manual Section 3: Prokaryotic Sample and array Processing (www.affymetrix.com/support/downloads/manuals/expression_s3_manual.pdf).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Yin X., Wu Orr M., Wang H., Hobbs E.C., Shabalina S.A, & Storz G. (2018). The small protein MgtS and small RNA MgrR modulate the PitA phosphate symporter to boost intracellular magnesium levels. Molecular microbiology, 111(1), 131-144.

Publication 2018

E. coli Genome 2.0 array

Arabinose Cdna Cells E coli Genome hybridization Prokaryotic

Corresponding Organization : Eunice Kennedy Shriver National Institute of Child Health and Human Development

Other organizations : Peking University, Chinese University of Hong Kong, National Institutes of Health, National Center for Biotechnology Information

Top 5 similar protocols

Variable analysis

independent variables

Expression of MgtS (pBAD24-MgtS vs. pBAD24)

dependent variables

Transcriptional changes (measured by Affymetrix E. coli Genome 2.0 array)

control variables

Growth of cells to OD600 ~0.5 in LB
Induction of cells with arabinose for 7.5 min

controls

Positive control: Cells harboring pBAD24-MgtS
Negative control: Cells harboring pBAD24

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!