Quantifying Apoptosis in Gastric Cancer Cells

Cell apoptosis was assessed as previous protocols (42 (link)). Gastric cancer cells were treated with ARV-825 of different concentrations. At 72 h after treatment, the cells were trypsinized, centrifugated at 180g for 4 min and washed with PBS. The cells were suspended in binding buffer and stained using the fluorescein isothiocyanate-Annexin V apoptosis kit (Cat. 556420, BD Biosciences). The cell apoptosis was tested and analyzed by a Beckman Gallios™ flow cytometer.

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Liao X., Qian X., Zhang Z., Tao Y., Li Z., Zhang Q., Liang H., Li X., Xie Y., Zhuo R., Chen Y., Jiang Y., Cao H., Niu J., Xue C., Ni J., Pan J, & Cui D. (2021). ARV-825 Demonstrates Antitumor Activity in Gastric Cancer via MYC-Targets and G2M-Checkpoint Signaling Pathways. Frontiers in Oncology, 11, 753119.

Publication 2021

fluorescein isothiocyanate-Annexin V apoptosis kit Gallios™ flow cytometer

After treatment Annexin v Apoptosis Arv 825 Buffer Cell Fluorescein Gastric cancer Isothiocyanate

Corresponding Organization : National Engineering Research Center for Nanotechnology

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Variable analysis

independent variables

ARV-825 concentration

dependent variables

Cell apoptosis

control variables

Time after treatment (72 h)
Cell line (gastric cancer cells)
Centrifugation (180g for 4 min)
Washing with PBS
Staining with fluorescein isothiocyanate-Annexin V apoptosis kit
Flow cytometry analysis

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