Data were acquired using an Agilent 6550 QToF instrument. Samples were ionized using an Agilent Jet Stream electrospray ionization source operated in negative mode. Gas temperature in the ion source was 150 °C with a flow rate of 14 L/min. Nebulizer pressure was 45 psig; sheath temperature was 325 °C with a gas flow rate of 12 L/min. Voltage for both capillary and nozzle was 2000 V. The funnel DC voltage was −30 V, funnel voltage drops were −100 and −50 V in the high- and low-pressure funnels, respectively. The RF voltages were 110 and 60 V in the high- and low-pressure funnels respectively. Mass spectra were acquired between 50 m/z and 1100 m/z with a rate of 2 spectra per second. Mass lock mixture was used as described in Wan et al.63 (link).
Other organizations :
SickKids Foundation, Hospital for Sick Children, Structural Genomics Consortium, University of Toronto, Universidade Estadual de Campinas (UNICAMP), Princess Margaret Cancer Centre, University Health Network, Institute for Research in Immunology and Cancer, Université de Montréal, Université de Sherbrooke, Ontario Institute for Cancer Research, University of Calgary, Eli Lilly (United States), St. Michael's Hospital
Agilent Jet Stream electrospray ionization source operated in negative mode
dependent variables
Mass spectra acquired between 50 m/z and 1100 m/z with a rate of 2 spectra per second
control variables
Gas temperature in the ion source at 150 °C
Gas flow rate of 14 L/min
Nebulizer pressure at 45 psig
Sheath temperature at 325 °C
Sheath gas flow rate of 12 L/min
Capillary voltage at 2000 V
Nozzle voltage at 2000 V
Funnel DC voltage at -30 V
Funnel voltage drops of -100 V and -50 V in the high- and low-pressure funnels, respectively
RF voltages of 110 V and 60 V in the high- and low-pressure funnels, respectively
controls
Mass lock mixture used as described in Wan et al. (2013)
Annotations
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