Histopathological Analysis of Tumor Tissues

Histopathological analysis of the collected tumour tissues was carried out using the Haematoxylin and Eosin (H&E) Staining Kit (#C0105S, Beyotime) according to the product instructions. Briefly, the tumour tissues were successively fixed in 4% PFA, dehydrated using gradient ethanol, embedded in paraffin, and sliced into 4 μm sections. The sections were then deparaffinized in xylene, hydrated in gradient ethanol, stained with haematoxylin for 2 min and eosin for 1 min. Finally, the sections were sealed with neutral resin. The results were observed and photographed with a microscope (30 (link)).

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Li Y., Xiong J.B., Jie Z.G, & Xiong H. (2022). Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta gene as a tumour suppressor in stomach adenocarcinoma. Frontiers in Oncology, 12, 1069875.

Publication 2022

Haematoxylin and Eosin (H&E) Staining Kit

Embedded paraffin Eosin Ethanol Microscope Resin Tissues Tumour Xylene

Corresponding Organization : Nanchang University

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Variable analysis

independent variables

Haematoxylin and Eosin (H&E) Staining Kit (#C0105S, Beyotime)

dependent variables

Histopathological analysis of the collected tumour tissues

control variables

4% PFA for tissue fixation
Gradient ethanol for tissue dehydration
Paraffin embedding
4 μm tissue sections
Xylene for deparaffinization
Gradient ethanol for tissue hydration
Haematoxylin and eosin staining
Neutral resin for sealing

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