Molecular Profiling of Neurodegeneration

Dulbecco's modified Eagle's medium medium (#12100046), fetal bovine serum (#26140079), penicillin/streptomycin (#15140122), OptiMEM (#31985062), Lipofectamine (#11668030), PBS (#14190), chemiluminescence kit (#32106), and Imperial protein stain (#24615) were purchased from Thermo Fisher. Th-S (T1892) and Th-T (T3516) were purchased from Sigma. Anti-ITM2B (sc-374362) was purchased from Santa Cruz; anti-ApoE (66830) from ProteinTech; anti-C1Q (ab182451) from Abcam; anti-FCRL4 (PA5-87813) from Invitrogen; anti-GFAP (G3893) from Sigma; and anti-Myc (sc-40) and anti-Tubulin (sc-23948) from Santa Cruz. Anti-ADan (ab 1700) polyclonal antibody has been reported previously (10 (link)).

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Fernandez A., Gomez M.T, & Vidal R. (2022). Lack of ApoE inhibits ADan amyloidosis in a mouse model of familial Danish dementia. The Journal of Biological Chemistry, 299(1), 102751.

Publication 2022

Dulbecco's modified Eagle's medium medium fetal bovine serum penicillin/streptomycin OptiMEM Lipofectamine PBS chemiluminescence kit Imperial protein stain Th-S (T1892) Th-T (T3516) anti-GFAP (G3893)

Antibody Apoe Chemiluminescence Fcrl4 Fetal bovine serum Gfap Lipofectamine Penicillin Protein Stain Streptomycin Tubulin

Corresponding Organization : Indiana University – Purdue University Indianapolis

Top 5 similar protocols

Variable analysis

independent variables

Th-S (T1892)
Th-T (T3516)

dependent variables

ITM2B
FCRL4
Tubulin

control variables

Dulbecco's modified Eagle's medium medium (#12100046)
Fetal bovine serum (#26140079)
Penicillin/streptomycin (#15140122)
OptiMEM (#31985062)
Lipofectamine (#11668030)
PBS (#14190)
Chemiluminescence kit (#32106)
Imperial protein stain (#24615)

positive controls

Anti-ADan (ab 1700) polyclonal antibody

negative controls

Not explicitly mentioned

Annotations

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