Optimizing Lipid Production in Pseudochlorella

For optimize the nutrient grown, employing different concentrations of (CO₂ and bicarbonate) as a carbon sources, bicarbonate at 2, 4 and 8 g L¹ and CO₂ enriched air supply 4, 8, 16% in two liter Erlenmeyer flasks each containing 1.80 L of BBM medium, was achieved, that to ensure the better results for accumulation high lipid contents. The cultures was grown photo-autotrophically under 10 white florescent light lamps (Philips 40 W) provided an illumination of 2500 lx. After the first steady-state condition was reached, CO₂ at 8% as a carbon source was selected due to given higher biomass dry weight and biomass productivity. Further treatments, by study the effect of the three elements availability (N, P, S) in BBM growth medium (at Lab scales) was determined to optimize and develop the economic viability of Pseudochlorella pringsheimii as feedstock biodiesel. Therefore, the algae was growing in different medium containing serial concentrations of nitrogen (KNO₃, 0.0, 0.125, 0.25, 0.50 g/L), phosphorus (KH₂PO₄, 0.0, 0.13, 0.175, 0.14 g/L), iron (FeSO₄, 0.0, 5.0, 10.0 mg/L) to enhance lipid yield and high lipid productivity. In all cultivated flasks, conductivity, salinity, pH and temperature were measured every two days with Hanna (HI 09,812–5, HANNA instruments, USA) conductivity meter. The purity of cultures was periodically checked by microscopic observation following taxonomy guidelines.